• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    1. DEVICE FOR CONDUCTING ANALYSIS containing a rotor with a set of peripheral equidistant cells from its axis with a readout window with a nozzle for entering the analyzed fluid, and radial channels connecting the cells with a device for dosing the analyzed fluid, an analyzer, npoi ib cell means , made in the form of a central nozzle, placed along the axis of rotation of the rotor and connected by means of radial channels with cells, in each of which a peripheral outlet of liquid outlet is made and placed A solid carrier, characterized in that, in order to simplify the maintenance of the device, it is provided with drain cups located on the periphery of the rotor, adjacent to the cells, each of which communicates with one drain cuvette and a reagent attachment mounted on the rotor.
    公开号:SU1085502A3
    申请号:SU813361533
    申请日:1981-12-09
    公开日:1984-04-07
    发明作者:Гиган Жан
    申请人:Guigan Jean；
    IPC主号:
    专利说明:

    2. The device according to claim 1, about tl and which is based on the fact that the cuvette is located above the cell.
    3, the apparatus according to claim 1, characterized in that the nozzle for injecting the analyzed liquid is mounted concentrically with the central nozzle and communicates with the drain cuvettes by means of the radial channels formed in the rotor.
    4. The device according to Claim 1, -o t that is based on the fact that the radial channels that connect the nozzle for the input of the analyzed fluid with the drain cells, are made with capillary 14 narrowing near the drain cuvette, the pocket and the shutter installed in the nozzle for the fluid .
    The invention relates to devices for analyzing, for example, biological liquids by a photo-gauge method.
    A device is known for analyzing an containing rotor with a set of peripheral equidistant cells from its axis with a readout window, with a port for entering the analyzed liquid and radial channels connecting the cells with the device for dosing the analyzed liquid, the analyzer, means for penetrating the cells made in the form of a center pipe placed along the axis of rotation of the rotor and connected by means of radial channels with cells, in each of which a peripheral outlet of liquid is made and the solid support is placed, for example in the form of a ball.
    The rotor is installed under the device for dosing with the calculation of input into each cell through the upper nozzles of a certain amount of composition such as serum, plasma or any other biological fluid containing the analyte; after this injection, the liquid required time is in the state of incubation. The rotor is then rotated through the center1: the washing port is introduced into the washing fluid. Means forming deflectors for guiding the protruding fluid to the bottom of the cells are provided at the outlet of the radial channels. With respect to each cell, centrifugation is intended to eliminate the excess composition not captured by the solid-state substrate through the peripheral hole.
    After washing, it is possible to proceed (if desired, to dry, stopping the access of the washing liquid and maintaining centrifugation. These operations are then repeated with a reagent, which tends to be fixed on the shT chain formed during incubation.
    After new incubation, elimination of unreciphered excess, washing, drying, proceed to reading with a counter if the reagent has been labeled with an isotope; if the labeling was performed with an enzyme, add a metered amount of the substance called a colored reaction, which is read directly into the cuvette using photometer l.
    A disadvantage of the known device is that to fill the cell with the composition and reagents, it must be installed in front of appropriate devices for dosing the analyzed liquid, such as a pipette, which limits its autonomy
    The aim of the invention is to simplify the maintenance of the device.
    The goal is achieved by the fact that a device comprising a rotor with a plurality of peripheral equidistant cells from its axis with a reading window, with a port for entering the analyzed liquid, and radial channels connecting the cells with a device for dosing the analyzed liquid, an analyzer, a means for washing the cells, made in the form of a central nozzle placed along the axis of rotation of the rotor and connected by means of radial channels to the cells, in which one the peripheral opening of the outlet is filled with liquid minute and the solid support is placed, is provided arranged around the periphery of the rotor drain ditches adjacent to the cells, each of which is in communication; with one drain cuvette, and a reagent attachment mounted on the rotor.
    In this case, the cuvette is located above the cell.
    In addition, a nozzle for injecting the liquid to be analyzed is mounted concentrically to the central nozzle and communicates with the drain cuvettes through radial channels formed in the rotor. Moreover, the radial channels / connecting pipe for entering the analyzed fluid with drain cells are made with a capillary constriction near the drain cell, pockets and a shutter installed in the pipe for fluid injection. FIG. 1 shows the proposed device with a partial cut-out at the level of a pair of adjacent interacting cells, top view; on Fig.2 the first version of the proposed device, a partial view in axonometry; in fig. 3 is a view A of FIG. 2; in fig. 4 shows a section BB in FIG. 3; on. FIG. 5 shows a section B-B in FIG. 3; in fig. 6 - a pair of cells, part of the proposed device, a partial view in axonometry; figure 7 view of the proposed device along its centerline; in fig. 8 - another variant of the proposed device, showing a pair of adjacent interacting cells, a partial perspective view; in fig. 9 - a pair of adjacent interacting cells, section, passage through radial feed channels of cells; in fig. 10 shows a section of YYY in FIG. 9; in fig. 11 is a section d-d in FIG. eight; in fig. 12 - the proposed device with a partial section of the central part of the device. The proposed analysis device contains a rotor 1 having a series of peripheral cells 2 for analysis, each of which contains a solid carrier 3 made in the form of a ball (for example, from polystyrene coated with a composition having antibody properties). Instead of one such ball, a series of small balls can be used, for example, having a diameter from 10 to 20 microns. In this case, a barrier is installed in the cells capable of holding balls in the cells during the rotation of the device, and this barrier is inserted. in the grooves or fitted into the corresponding grooves. It is advantageous to install such barriers such as a molecular filter. A solid carrier may also be formed by an appropriate coating applied to the lower part of the cell wall. The central nozzle 4 serves to supply the washing fluid, and radial channels 5 emerge from it connecting the central opening with each peripheral cell 2. The channels are located in a plane perpendicular to the axis of rotation of the device. The radial channels 5 are formed by two complementary discs 7 and 8 (Fig. 3), but there can be any other variant of the channel, such as a flat lip on one rotor disk and a notch on the other. Each cuvette also has a flange-shaped peripheral hole 9 for discharging fluid, which is formed by overlapping the peripheral edges of coaxial discs 7 and 8 forming the rotor 1. This outlet ends at a drainage chute 10 located in the rear part of the cell relative to the direction of rotation, during the entry of the washing liquid into the cuvette, for example, clockwise. A nozzle 11 for introducing the analyzed liquid is mounted concentrically to the central nozzle 4 and is connected via radial channels 12 to drain cuvettes 13 adjacent to cells 2. Each cell 2 is connected to one drain cuvette 13 and has a common wall 14 (Fig. 1-8). Channels reporting each cell 13 with cell 2 are located at wall 14. In the first embodiment of the proposed device, the common wall 14 is placed radially, in the second wave, the radiant cuvette 15 is located above the cell 2, and the common wall 16 is formed by the upper surface of the cuvette. The radial channels 12 in the vicinity of the drain cuvettes are made with a capillary narrowing 17, with a pocket defining a predetermined volume of the bolt inserted into the hole, and with a bolt (not shown). Radial channels 12 may also be formed by complementary portions formed in the rotor components, but any other type of channel may also be present. On the upper disc 7 of the rotor, a device for reagents is fixed, made, for example, in the form of capsules, ampoules, containing reagents and positioned so that, as a result of centrifuging and opening these vessels, the liquid contained in them. , the bones are inserted into the drain cells 13 or 15. In the first embodiment of the device of FIG. 1-7), the device 18, made in the form of vessels 19 and 20, contains. reagents. The vessels on the disc 7 are held by the blocking supports 21. The adjacent cells 2 and the cuvettes 13 are closed in the upper part by the cover 22, which has an opening 23. The analysis device has means (not shown) allowing the vessels 19 and 20 to be opened and operating by radiation, heating or crushing. In the second version of the device (Fig. 8-12), the device 18 is made in the form of vessels 24 held in cells 25, the output hole of pcfiHe 26 of which is located at the level of the elongated part 27. Before opening, the end of part 27 comes into contact with the corresponding hole 28 in the front wall of cell 25. When they want to open the vessel 24, turn the back part down along c: trelka and cause destruction of the elongated part 27, thus forming the hole 26. The reagent flows out of the hole 26 and passes into the opening 29, made in the corner 30 slime Tsoi cuvettes 15, K The cap 30 has a small visor 31, which provides the direction for the supply of liquid from the vessel 4 to the drain cuvette 15. Any other appropriate means can be used to open the vessels containing reagents and fix them on the upper surface of the rotor. the wall of the drain cuvette and chek ek has a hole that provides a passage between them (in the first embodiment, this hole 32, and in the second one, the opening hole 33). The internal profile of the cuvette, in both variants, is such that the liquid entering these cuvettes during centrifugation is retained in them without the possibility of going into cells 2 (containing solid media 3) a centrifugation time in the direction, for example, counter clockwise, when By ceasing centrifuging, all the liquid located in the drain cuvette 13 or 15 passes into the cell 2 through the opening 32 or 33, respectively. For this purpose, the opening 32 is located at the level reached by the liquid in the cell 2 The cuvette 13 is kept in a cuvette 13. The cuvette 13 for draining (Fig. b) has in its outer side a holding area 34 of a protruding drain slope 35 located at a level below the opening 32. Thus, after termination of the centrifuge, the liquid that was held in zone 34 is drained into the cell 2 through an inclination of 35 and the opening 32 of the Cuvette 15 located above the cell 2. And the discharge of the liquid that is in it, when the centrifugation is stopped, does not create any problems. Near the end of the supply radial, channel 5, leaving the cell. 2, a reflector 36 is provided, which guides the flushing liquid to the bottom of the cell 2. The rotor 1 is driven to rotate by a drive (not shown) through the shaft 37 to the bearing lug 38, which engages with the corresponding rotor seat 39. The device works as follows. The liquid containing the analyte is introduced into the concentric port 11 into the channel 12. Then proceed to centrifugation in a direction, for example, counterclockwise. The liquid, having the analyte contained in the channels 12, is then introduced into the drain cups 13 or 15 and remains there until centrifugation is active. When the centrifugation is stopped, the liquid penetrates the cells 2 containing the solid carrier 3 and is left at rest for the necessary incubation period. It should be noted that each cell 2 has sufficient volume to place the ball and the liquid with the analyte without the risk that the liquid level reaches the channels 5. After this phase of capture by the antigen-antibody reaction, excess liquid is removed by centrifuging in a clockwise direction, entering through the channels 5, the washing liquid (water) entering the central nozzle 4. This liquid washes the solid carrier and flows out, like an excess of liquid, with the analyte, through an opening 9 located at the end e drain chute 10, Then, if necessary, proceed to the stage of drying, stopping the access of industrial liquid and continuing centrifugation. Then proceed to the introduction of the first reagent contained in the vessels installed on the upper disc 7 of the rotor, for example polyglucoside. For this, centrifugation is performed in a counterclockwise direction and the opening of the reagent vessel is opened. The liquid flows out of the vessel into the drain cuvette 13 or 15, where it is held for as long as it is centrifuged in the indicated direction. When the rotation is stopped, the reagent passes from the overflow cuvette 13 or 15 to the cell 2 for stalting and comes in contact with the Solid carrier modified as a result of the previous reaction of the antigen-antibody. After a new incubation period, removal of uncovered excess, washing, drying in accordance with the nature of the reagent introduced, you can proceed directly to the analysis, for example, using a Geiger counter, if the reagent contained raoisotopes, or enter a second reagent in the manner described, for example, caused the colored reaction , allowing to measure the enzymatic activity using a colorimeter or photometer. To facilitate this type of analysis, it is advantageous for the inner and outer walls of cell 2 to be parallel for analysis at least at the level of the meter reading window 40.
    In the case where the solid carrier is in the form of a sharlek, support means 41 are provided in the analysis cell so that the ball does not obstruct the reading windows. A solid carrier that is in different cuvettes for analysis may correspond to different analyzes that are carried out simultaneously on the basis of the same sample.
    /five
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    The proposed device allows you to quickly and easily produce five simultaneous quantitative angles of information required for the analysis of the thyroid. Flexibility is manifested, along with others, in the possibility of dividing the rotor into two zones for using some - cells for analyzing control standards, and placing only identical solid carriers, which allows the same test to be made for many customers, or different values of dx. The proposed device can be implemented in very small dimensions. It may have, for example, a diameter of about 8 cm.
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    权利要求:
    Claims (4)
    [1]
    1. ANALYSIS DEVICE, comprising a rotor with a plurality of peripheral cells equally spaced from its axis with a readout window, a nozzle for introducing the analyzed liquid, and radial channels connecting the cells with a device for dispensing the analyzed liquid, an analyzer, a means for washing the cells, made in the form of a central nozzle placed along the axis of rotation of the rotor and connected by means of radial channels to cells, in each of which a peripheral hole for the liquid outlet is made and a TV is placed rdy carrier, characterized in that, in order to simplify maintenance device is provided with a circumferentially disposed drain cuvette rotor, adjacent to the cells, each of which communicates with a drain cuvette and hardened on the rotor device for reagents.
    [2]
    2. The device according to p. ^ Characterized in that the cuvette is placed above the cell.
    [3]
    3. The device according to π. 1, characterized in that the nozzle for introducing the analyzed liquid is mounted concentrically to the central nozzle and communicates with the drain cells through radial channels made in the rotor.
    [4]
    4. The device according to claim 1, wherein the radial channels communicating the pipe for introducing the analyzed fluid with drain cells are made with capillary constriction near the drain cell, pockets and a shutter installed in the pipe for fluid injection.
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    引用文献:
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    US4817453A|1985-12-06|1989-04-04|E. I. Dupont De Nemours And Company|Fiber reinforced centrifuge rotor|
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    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    FR8026528A|FR2496268B1|1980-12-15|1980-12-15|
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